Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: TRAF2 and NCK interacting kinase: a novel regulator of integrin α IIb β 3 signaling in platelets

doi: 10.1016/j.rpth.2025.103204

Figure Lengend Snippet: KY-05009 impairs platelet tethering to immobilized fibrinogen. Adhesion of (A) vehicle (0.5% v/v dimethylsulfoxide [DMSO]) or (B) KY-05009 (10 μM)-treated platelets (4 × 10 7 cells/mL) to fibrinogen (100 μg/mL). F-actin was stained with Alexa Fluor 568 phalloidin (1:100 dilution). Platelets were visualized with a Nikon Eclipse Ts2 Fl microscope using a 40X/0.65 objective. Representative images are shown: (C) percentage platelet coverage and (D) mean data; n = 4. Data are presented as mean ± SEM. Data analyzed by 1-way ANOVA; ∗ P < .03.

Article Snippet: Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective.

Techniques: Staining, Microscopy

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: TRAF2 and NCK interacting kinase: a novel regulator of integrin α IIb β 3 signaling in platelets

doi: 10.1016/j.rpth.2025.103204

Figure Lengend Snippet: KY-05009 treatment attenuates thrombus formation under arterial shear stress. DIOC6 labeled whole blood was treated with (A) vehicle (DMSO, 1% v/v), (B) KY-05009 (10 μM), (C) KY-05009 (50 μM), or (D) KY-05009 (100 μM) and perfused over collagen coated (100μg/mL) Vena8 Fluoro+ Biochips using a Cellix perfusion system at arterial sheer stress (20 dynes/cm 2 ) for 10 minutes. Thrombus formation was determined by monitoring the change in fluorescence intensity observed over recorded intervals. Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective. The fluorescence intensity of the formed thrombi were normalized to the maximum fluorescence intensity value of the vehicle after 10 minutes and expressed as a percentage; n = 6. Decimated averages at each time point are presented ± SEM. (E) Annexin V binding to platelets treated with vehicle (DMSO, 1% v/v) or KY-005009 (10-100 μM). Platelets were gated and 10 000 events were recorded. Mean ± SEM of raw median fluorescence intensity data are presented; n = 3. Thrombus formation data analyzed by 2-way ANOVA; flow cytometry data analyzed by 1-way ANOVA; ∗ P < .03.

Article Snippet: Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective.

Techniques: Shear, Labeling, Fluorescence, Microscopy, Binding Assay, Flow Cytometry